Enhancement of mucosal enteroviral DNA vaccine immune response with co-immunization with Flt3L or GM-CSF gene
Date Issued
2005
Date
2005
Author(s)
Lu, Yen-Yi
DOI
zh-TW
Abstract
Enterovirus 71(EV71) has been the most important enterovirus to cause the high fatality and severe neurological diseases. Due to the fact that there is still no appropriate treatment to remedy patients after they are infected, efficient DNA vaccine might be an effective approach to induce immune response against EV71 infections. Immune responses generated by DNA vaccination are initiated by antigen-presenting cells (APCs), especially dendritic cells. Both FMS-like tyrosine kinase 3 ligand(Flt3L) and granulocyte-macrophage colony stimulating factor(GM-CSF) proteins play crucial roles in the development and expansion of dendritic cells, the professional APCs. In this study, we immunized female Balb/c mice with EV71 vp1 plasmid DNA and co-immunize with or without Flt3L or GM-CSF genes as the adjuvant genes by intranasal administration. We demonstrated that immunization with vp1 plasmid DNA accompanies with Flt3L adjuvant gene via mucosal (intranasal) route could induce higher anti-VP1 specific IgA production. Balb/c mice immunized with vp1 and Flt3L plasmids had much higher seral IgA titer than that of untreated group. Our result indicated that the combination of Flt3L gene with enteroviral vp1 gene immunization enhanced antibody production and triggered a better mucosa immune response for protection from enteroviral infection.
Subjects
疫苗
腸病毒
vacciine
enterovirus
Flt3L
GM-CSF
SDGs
Type
other
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