Functional Assay of php-3 in Cell Corpse Engulfment in C. elegans
Date Issued
2006
Date
2006
Author(s)
Li, Chi-Hua
DOI
zh-TW
Abstract
Programmed cell death (PCD) plays an important role in the development of multicellular organisms. After cells undergo PCD, the cell corpses are recognized and engulfed by engulfing cells. In C. elegans, there are two partially redundant pathways for engulfment: ced-1/ced-6/ced-7/dyn-1, and ced-2/ced-5/ced-12. These two pathways converge at ced-10. During apotosis, phosphatidylserine (PS), which is normally restricted to the inner leaflet of the plasma membrane, is exposed on the surface of apoptotic cells and act as an “eat me” signal to trigger phagocytosis. In C. elegans, the phosphatidylserine receptor PSR-1 binds cells with exposed PS and acts upstream in the ced-2/ced-5/ced-12 pathway (Wang et al., 2003). In this study, we found that PHP-3, a posterior Hox protein, can interacts with both intracellular and extracellular region of PSR-1. Like psr-1 embryos, php-3 mutants have more corpse number and longer cell-corpse-duration time than wild type. Genetic analysis of psr-1; php-3 double mutants suggest that psr-1 and php-3 likely act in the same pathway during programmed cell death. Double mutant analysis with previously identified engulfment genes showed that the php-3 mutation reduced the persistent corpse number of ced-1, ced-2 and ced-7 mutants, but not ced-5, ced-6, ced-10 and ced-12 mutants, suggesting that php-3 may have antagonistic effect with ced-1, ced-2 and ced-7.
Subjects
計畫性細胞死亡
吞噬
programmed cell death
engulfment
Hox gene
php-3
Type
other
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