In Vitro Test for the Multiple Transgenic Pig Cell
Date Issued
2002-07-31
Date
2002-07-31
Author(s)
李章銘
DOI
902314B002429
Abstract
Pig to human xenotransplatation is a promising strategy to overcome the organ shortage in
clinical transplantation. However, the application is hampered by the xenoreactive antibody
mediated hyperacute rejection, delayed xenograft rejection and MHC disparity associated T cell
rejection. Previous, we have produced the HLA DPW0401 transgenic pig, which was shown
equipped with the human HLA antigenecity and, as compared to the non-transgenic litermate pig,
induced a minor cellular response to the human PBMCs that came from the HLA DPW 0401(+)
human. In this study, we tried to evaluate whether there is similar effect on human to pig xenogenic
cellular response for the cells from transgenic pig of HLA DQw 0601. We used direct xenogenic
mixed lymphocyte culture to see whether the human –to-pig xenogenic cellular response is
attenuated by the trasngenosis of HLA DQ0601 on the porcine cells. The human anti-porcine Th2
response was evaluated by the production INFγin the culture media. Results：The cellular
proliferation of human PBMC under the stimulation of porcine PBMC was reduced by the presence
of HLA DQ molecules on the porcine cells as compared to that of the non-transgenic littermate
control. The human to porcine xenogenic Th1 response, as represented by the production of INF
γ , was also attenuated in the HLADQ transgenic pig cells. These phenomenons can be
demonstrated in the human PBMCs with or without the HLADQw0601 allele. This study on the
HLA DQw0601 transgenic pig further support the concept that increasing the similarity of MHCII
between pig and human in the HLA transgenic pig can help to reduce the xenogenic cellular
response between human and pig.
Subjects
異種移植（Xemo transplantation）
HLA
豬（Pig）
Publisher
臺北市：國立臺灣大學醫學院外科
Type
report
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