Ultrasonically extracted £]-D-glucan from artificially cultivated mushroom, characteristic properties and antioxidant activity
Journal
Ultrasonics Sonochemistry
Journal Volume
35
Pages
531-540
Date Issued
2017
Author(s)
Abstract
Ganoderma mushroom cultivated recently in Malaysia to produce chemically different nutritional fibers has attracted the attention of the local market. The extraction methods, molecular weight and degree of branching of (1-3; 1-6)-£]-D-glucan polysaccharides is of prime importance to determine its antioxidant bioactivity. Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-£]-D-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma. The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-£]-D-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods. ? 2016 Elsevier B.V.
Subjects
Antioxidant
Extraction
Ganoderma
Polysaccharides
Ultrasound
£]-D-Glucans
SDGs
Other Subjects
Antioxidants; Bioactivity; Molecular weight; Polysaccharides; Toxic materials; Ultrasonics; Anti-oxidant activities; Characteristic properties; Ganoderma; Glucans; Mon-osaccharides compositions; Physical characteristics; Structural characteristics; Ultrasound-assisted extraction; Extraction; antioxidant; ascorbic acid; beta 1,3 glucan; beta 1,6 glucan; Ganoderma lucidum extract; hot water; monosaccharide; phenol derivative; 1,1-diphenyl-2-picrylhydrazyl; antioxidant; biphenyl derivative; glucan; monosaccharide; picric acid; antioxidant activity; Article; controlled study; cultivated species; drug isolation; drug structure; energy consumption; extraction and extracts; Ganoderma lucidum; gel permeation chromatography; in vitro study; infrared spectroscopy; Malaysia; molecular weight; nonhuman; priority journal; Soxhlet extraction; ultrasound assisted extraction; Agaricales; chemistry; isolation and purification; ultrasound; Agaricales; Antioxidants; Biphenyl Compounds; Glucans; Molecular Weight; Monosaccharides; Phenols; Picrates; Ultrasonic Waves
Type
journal article