Pbrm1 Loss Induces a Permissive Chromatin State for Cholangiocytic Differentiation and Cholangiocarcinoma Formation
Journal
Cellular and Molecular Gastroenterology and Hepatology
Series/Report No.
Cellular and Molecular Gastroenterology and Hepatology
Journal Volume
20
Journal Issue
6
Start Page
101720
ISSN
2352-345X
Date Issued
2026
Author(s)
Chiou, Li-Wen
Jhuang, Yu-Lin
Hsu, Chia-Lang
Cheng, Yen-Hsu
Lee, Chia-Hsiang
Fang, Yi-Ting
Jeng, Yung-Ming
Abstract
Background & aims: The SWI/SNF ATP-dependent chromatin remodeling complex regulates transcriptional machinery access and is critical in normal physiology and cancer development. PBRM1, a key subunit of this complex, is frequently mutated in intrahepatic cholangiocarcinoma (iCCA). This study aims to explore the role of PBRM1 in liver physiology and its involvement in iCCA development.
Methods: Liver-specific Pbrm1 knockout (Pbrm1 KO) mice were generated to assess the effects of Pbrm1 loss under various conditions. These mice were exposed to a 3,5-diethoxycarbonyl-1,4-dihydrocollidine diet to induce cholestatic injury and were also subjected to a high-fat diet to evaluate susceptibility to liver steatosis. Chromatin accessibility and gene expression under both normal and injury conditions were examined. Additionally, the impact of Pbrm1 loss was analyzed in combination with an activating KrasG12D mutation to study cancer development.
Results: Pbrm1 KO mice exhibited increased susceptibility to cholestatic injury, with an enhanced ductular reaction. Loss of Pbrm1 reduced chromatin accessibility at hepatocyte-specific and metabolically important genes, although RNA expression remained unaffected during homeostasis. Following cholestatic injury, hepatocyte-specific gene expression was significantly reduced compared with wild-type controls. Pbrm1 KO mice also showed heightened vulnerability to high-fat diet-induced liver steatosis. When combined with KrasG12D mutation, Pbrm1 KO/KrasG12D mice had shorter survival and were more likely to develop cholangiocarcinomas, whereas Pbrm1 wild type/KrasG12D mice predominantly developed hepatocellular neoplasms. PBRM1-deficient organoids were highly sensitive to the EZH2 inhibitor tazemetostat, whereas effects on allografts were limited.
Conclusions: PBRM1 maintains chromatin accessibility for hepatocyte differentiation-related genes. Its loss promotes differentiation toward cholangiocytes during injury or tumorigenesis, driving iCCA development.
Subjects
Cell Fate
Chromatin Remodeling
Knockout Mice
SNF/SWI Complex
Publisher
Elsevier BV
Type
journal article