Variation of Simple Sequence Repeated DNAs and their Flanking Regions among Legume Crops
Date Issued
2005
Date
2005
Author(s)
Yang, Sin-jia
DOI
zh-TW
Abstract
To study genetic variation among legumes, ISSR and SSR DNA markers were analyzed around simple sequence repeats and their flanking regions for (1) the variation of simple sequence repeated DNAs and their flanking regions among legume crops (2) the feasibility of applying SSR and ISSR DNA markers in different species of legumes (3) the phylogenetic analysis of legume crops. Twelve important legume crops (33 varieties), including soybean (Glycine max), peanut (Arachis hypogaea), small red bean (Vigna angularis), mungbean (V. radiata), cowpea (V. unguiculata), pea (Pisum sativum), broad bean (Vicia faba), jack bean (Canavalia ensiformis/ gladiata), common bean (Phaseolus vulgaris/ coccineus) and pigeon pea (Cajanus cajan), were investigated. The transferability of SSR DNA markers was also used to evaluate the genetic similarity between the core species (soybean), which the SSR primesrs were originally designed, and other species. The conservative in primer annealing regions was found in pea and soybean. The experimental results indicate that it is applicable of using SSR markers of a species for the other legumes. In this study, two primer pairs able to amplify SSR sequences for 12 legume crops and rice were discovered. Also, lots of DNA sequence variations were identified among the similar length amplicons of the legume crops. And the distant species could be by discriminated by merely using DNA sequences amplified by soybean AG81 primer pairs. Because of the divergence in size of legume genomes, a great deal of ISSR primers were identified for a specific crop. These primers are available for detecting genetic variation among or within legume crops at molecular level. Because the long genetic distance among small red bean, mungbean and cowpea was found in the genetic similarity, cluster analysis and principle component coordinate analysis, each of the three species was suggested to become an isolated genera. According to SSR DNA and ISSR analysis, the broad bean had the largest genetic distance to the other legume crops. The finding of the degenerated repeated sequence in the AG81 segments of the broad bean also indicated that lots of DNA sequence variation had occurred in the region. Results from this study not only have proved the usefulness of ISSR DNA markers in detecting genetic variation among legume crops, but also have demonstrated the potential of applying SSR DNA markers in the other species.
Subjects
豆類作物
簡單重複序列
Legume Crops
SSR
ISSR
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-94-R92621116-1.pdf
Size
23.31 KB
Format
Adobe PDF
Checksum
(MD5):cdd4a8297b8f633493528f28ee589eda