Proteome Changes in Caco-2 Cells Treated with Monascus-Fermented Red Mold Rice Extract
Resource
Journal of Agricultural and Food Chemistry 55 (22): 8987-8994
Journal
Journal of Agricultural and Food Chemistry
Journal Volume
55
Journal Issue
22
Pages
8987-8994
Date Issued
2007
Date
2007
Author(s)
Abstract
Monascus-fermented red mold rice has been extensively used as a folk medicine for thousands of years. Monascus secondary metabolites, including monacolin K, monascorubrin, and ankaflavin, have been reported to have an antiproliferative effect on cancer cells. However, the cell machinery responsible for the antiproliferation of Monascus-fermented red mold rice treatment in cancer cells remains unclear. A proteomic approach using two-dimensional gel electrophoresis, matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass spectrometry, and tandem mass spectrometry was used to identify proteins with modified expression in Caco-2 cells treated with Monascus-fermented red mold rice extract. A total of 20 proteins were identified with significantly altered expression (P < 0.05) in response to Monascus-fermented red mold rice extract treatment. The deregulated proteins that were identified included heat shock protein 70, protein kinase C ε type, clusterin-associated protein 1, and two tumor suppressors (N-chimaerin and calponin-2). Our results suggested the involvement of heat shock protein 70-mediated cytotoxicity in the Caco-2 cells treated with Monascus-fermented red mold rice extract. ? 2007 American Chemical Society.
Subjects
Anticancer; Monascus; Polyketides; Protein extraction; Proteomics
SDGs
Other Subjects
plant extract; proteome; tumor protein; amino acid sequence; article; cell division; cell strain CACO 2; chemistry; DNA fragmentation; drug effect; fermentation; human; isolation and purification; metabolism; microbiology; molecular genetics; Monascus; rice; Amino Acid Sequence; Caco-2 Cells; Cell Division; DNA Fragmentation; Fermentation; Humans; Molecular Sequence Data; Monascus; Neoplasm Proteins; Oryza sativa; Plant Extracts; Proteome; Monascus
Type
journal article
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