小鼠卵母細胞及早期胚發生時母源性及胚源性基因之表現及其扮演之生物功能(1/3)
Date Issued
2002
Date
2002
Author(s)
鄭登貴
DOI
902313B002303
Abstract
In this present study the expression of
mouse maternal and zygotic genes was
compared and identified using two strategies
based on differential display and expression
sequences tags (EST) against samples from
freshly ovulated mouse oocytes and 8-cell
embryos, respectively.
For mRNA differential display studies, a
total 30 combinations of arbitrary with anchor
primers were used and results appeared that of
the 20 genes identified, 17 zygotic genes were
found to be specifically expressed in embryos
only if they had reached to 2- ~ 4-cells or even
2
at much advanced stages. Whereas, expression
of the other three genes were characterized as
the maternal ones found only in those ovulated
oocytes before ferilization. One of the mOSG
(mouse oocyte specific gene; Gene bank
accession number: AF313913) cDNA was
detected only in mouse oocytes and showed a
high sequencing homology with the mouse
OM2a and OM2b, and may be a novel member
of this gene family. By further rapid
amplification 5’-end (RACE) techniques, we
obtained the full-length cDNA with 875 bp to
encode 105 amino acids. RT-PCR and Northern
blotting analyses demonstrated that the mOSG
mRNA was expressed merely in the mouse
ovary. Further localization of transcripts using
in situ hybridization confirmed that expression
of mOSG occurred only in matured oocytes in
antrum follicles. On the other hand, using the
mouse EST-based primer-sets for RT-PCR, the
mZFG-1 (Zinc finger protein-1) gene was
identified and this gene was further confirmed
being expressed specifically in mouse
pre-implantation embryos (between 1-cell and
blastocyst stages). Based on the fact that
increase in amount of mZFG-1 gene transcripts
along the stages of embryos from 1-cell to
8-cells and subsequently followed by decrease
in its transcription activity suggested mZFG-1
gene may play an important role related to early
development and/or early differentiation events.
Subjects
mouse embryo
maternal gene
zygotic gene
gene expression
Publisher
臺北市：國立臺灣大學動物科學技術學系暨研究所
Type
report
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