Structural determination of immunoactive polysaccharides from Antrodia cinnamomea
Date Issued
2010
Date
2010
Author(s)
Zhan, Shih-Hao
Abstract
Antrodia cinnamomea is used as traditional Chinese medicine. In previous studies, polysaccharides from A. cinnamomea possess immune-modulating activity, and the structure determination is still not clear.
Mycelia of A. cinnamomea were first extracted with acetonitrile and followed with hot and cold water, in which the extract was further separated by gel filtration chromatography to give three fractions containing polysaccharides HMPS, LT70, and GT70. Due to the biological activities and amounts of polysaccharides, LT70 was selected for further purification and identification. By using ion exchange chromatography, LT70 was separated into three polysaccharide fractions; QA, QB, and QC.
In the present study, A. cinnamomea polysaccharides participated in the stimulation of nitric oxide(NO) production and stimulation of murine wild-type macrophages predominantly in tumor necrosis factor-α (TNF-α) and interleukin-6(IL-6) production.
From sugar analysis by gas-chromatography mass spectrum(GC-MS), the major components of A. cinnamomea polysaccharides QB are mannose and galactose in the molar ratio of 4:1. The main glycosidic linkages were also characterized by GC-MS. The results showed that there are terminal mannopyranose, 1,2 linked galactopyranose, 1,3 linked mannopyranose, and 1,2,6 linked mannopyranose. The structures of bioactive polysaccharides from A. cinnamomea were determined by chemical and NMR methods, and we preliminarily presume that the structure is as following:
And D might be a 6 linked monosaccharide to A or C as a branched terminal residue.The completed structure must be determined by further analysis.
Subjects
Antrodia cinnamomea
polysaccharides
immunoactivity
gel filtraton
GC-MS
TNF
NO
Type
thesis
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