行政院國家科學委員會補助專題研究計畫成果報告:細胞凋零素重組腺病毒於犬乳房腫瘤治療之研發(I)
Date Issued
2002
Date
2002
Author(s)
闕玲玲
DOI
902313B002310
Abstract
VP3 protein of chicken anemia virus has
been reported to induce apoptosis in
transformed and malignant cells but not in
normal diploid or primary cells. This
tumor-specific selective killing activity of
VP3 makes it an attractive candidate for
cancer gene therapy. To explore the
possibility of using VP3 protein as an
anti-tumor agent in veterinary medicine, a
cloned VP3 gene from a Taiwanese isolate
was constructed into eukaryotic expression
vectors and its effect on the cells originated
from a canine mammary gland tumor was
studied. The protein was first found
distributed in cytoplasm and later aggregated
in the nuclei. Cells expressing VP3 protein
underwent morphological changes and died
at a later stage after transfection. The cells
which expressed VP3 protein showed
apoptosis specific changes, e.g. shrinkage of
cells, segmentation of the nucleus,
condensation and degradation of DNA at 72
hours posttransfection. Taken together, the
data demonstrate that VP3 may constitute a
promising agent for the treatment of canine
mammary gland tumor.
Subjects
chicken anemia virus
VP3
protein
protein
apoptosis
gene therapy
SDGs
Publisher
臺北市：國立臺灣大學獸醫學系暨研究所
Type
report
File(s)![Thumbnail Image]()
Loading...
Name
902313B002310.pdf
Size
2.12 MB
Format
Adobe PDF
Checksum
(MD5):ad5f9c5a1f5bd37bcfb4954e13801295