Nanostructured mesoporous gold biosensor for microRNA detection at attomolar level
Journal
Biosensors and Bioelectronics
Journal Volume
168
Start Page
112429
ISSN
09565663
Date Issued
2020
Author(s)
Masud, Mostafa Kamal
Na, Jongbeom
Malgras, Victor
Preet, Anant
Ibn Sina, Abu Ali
Wood, Kathleen
Billah, Mutasim
Kim, Jeonghun
You, Jungmok
Kani, Kenya
Whitten, Andrew E.
Salomon, Carlos
Nguyen, Nam-Trung
Shiddiky, Muhammad J.A.
Trau, Matt
Hossain, Md Shahriar A.
Yamauchi, Yusuke
Abstract
Advances in nanoarchitectonics enable a wide variety of nanostructured electrodes with tunable shapes and surface for constructing sensitive biosensors. Herein we demonstrate the fabrication of a mesoporous gold (Au) biosensor for the specific and sensitive detection of miRNA in a relatively simple and portable manner. The electrocatalytic activity of the mesoporous Au electrode (MPGE) towards the redox reaction of Fe(CN)6]3-/4- expansively examined. Leveraging the electrocatalytic activity and signal enhancement capacity of the MPGE, an ultrasensitive and specific electrochemical sensor was developed for the detection of microRNA (miRNA). The target miRNA from spiked samples is selectively isolated and purified using magnetic bead-capture probe followed by the direct adsorption on the MPGE through direct affinity interaction between miRNA and mesoporous Au surface. The MPGE-bound miRNA is then quantified by differential pulse voltammetry (DPV) using [Fe(CN)6]4-/3- redox system (Faradaic current decrease with reference to the bare MPGE). This method evades the cumbersome PCR (polymerase chain reaction) and enzymatic amplification steps. This is a single-step assay building which can detect a wide dynamic linear range (100 aM to 1 nM) of miRNA with an ultra-low limit detection of 100 aM and present high translational potentiality for the development of high-performance detection tools for clinics. © 2020 Elsevier B.V.
Subjects
Biosensor
Electrocatalytic activity
Electrochemical detection
Mesoporous gold electrode
microRNA
Nanoarchitectonics
Publisher
Elsevier Ltd
Description
論文編號: 112429
Type
journal article