Development of the Multiplex PCR Assays for Mouse Parvovirus (MPV) and Minute Virus of Mice (MVM), and the Surveillance of MPV and MVM Infection in Laboratory Mice in Taiwan
Date Issued
2010
Date
2010
Author(s)
Wang, Ko-Wei
Abstract
Mouse parvoviruses are among the highly prevalent infectious pathogens in contemporary mouse colonies. There are two serotypes, minute virus of mice (MVM) and mouse parvovirus (MPV). Mouse parvoviruses have a predilection for mitotically active cells and can interfere with immunology, transplantation, and oncology research through virus-infected rodents, contaminated cells and biological materials. In order to detect these two viruses simultaneously, a multiplex PCR assay that amplifies the VP gene of MPV and MVM, and a mouse housekeeping gene has been developed in this study. The MPV/MVM/Actin Multiplex PCR assay specifically detects MPV and MVM, but doesn’t amplify KRV, RMV-1, RPV-1c, and H-1. The multiplex PCR assay could simultaneously detect both MVM and MPV in as low as 50 copies in the condition of equal-amount dual infection. The sensitivity of this multiplex PCR assay remained to detect at least 50 copies of MPV when the copies of MVM were 200 times higher than the MPV copy number, and vice versa. The prevalence surveillance result revealed that 3 out of 7 laboraotry mouse colonies were contaminated with MPV with the prevalence of 11.5%; however, none of the tested mouse colony was contaminated with MVM. The MPV/MVM/Actin Multiplex PCR assay developed in this study can provide a useful tool in the mouse health monitoring in the future.
Subjects
mouse parvovirus
minute virus of mice
MPV/MVM multiplex PCR
MPV prevalence
MVM prevalence
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