Biological, molecular and symptom determinater analysis of Cucumber mosaic cucumovirus isolates associated with banana mosaic disease
Date Issued
2007
Date
2007
Author(s)
Chou, Chun-Nan
DOI
en-US
Abstract
Abstract
Banana samples with mosaic symptoms were collected from southern Taiwan, and 12 type isolates of Cucumber mosaic cucumovirus associated with banana mosaic disease were established. The 12 CMV isolates were characterized into three types, (I-III), by the symptoms induced by CMV on bananas. The type I-infected bananas initially showed chlorosis with little necrosis streak, and later the full expanded leaves showed severe green mosaic with distortion either becoming slender leaf. Type II induced yellow mosaic with severe necrosis on leaves. The severe necrosis on pseudostem will eventually cause heart rot in field. Type III induced leaf chlorosis, however, the full expanded leaves show severe green mosaic without leaf deformation. For biological characterization of the collected CMV, we followed the methods described by Wahyuni (1992) and Wu (1994) using cowpea and N. glutinosa as differential hosts. When Nicotiana. glutinosa was used as differential hosts, Type I CMV induce severe green mosaic and parkling, or fern leaf-like leaf distortion. Type II CMV induce chlorosis on newly emerging leaves, and later the newly expanded leaves were deformed and caused necrosis spot on shoot tip and leaves. Type III only induce mild mottling and did not induce leaf distortion. When cowpea was used as the differential hosts, most Type I and Type II isolates can not systematically infect cowpea (belonging to as Solanaceae common strains). One isolate of type I (sm1) and the only isolate of type III (PL) can systematically infect the cowpea showing mosaic, (belonging to Legume strain). The phylogenetic analysis using CMV-CP sequences revealed that Legume and Solanaceae common strains were distinct groups. In addition to previously identified subgroups CMV IA, IB and II, new subgroups IS, IC and IT were confined. Members within these groups were geographic related, which suggest founder effect play roles in CMV evolution. One satellite RNA associated with isolate 22 without causing attenuate symptom on banana and N, glutinosa was identified. During characterization of CMV by differential host, isolates 20 caused stunting by repressing the internodes growth of N. benthamiana. By pseudorecombination between CMV 20 and 25 isolates the symptom determinater was found locating on RNA 2 of CMV 20. Following express the CMV proteins 2a and 2b protein individually or in combination, we found that the symptom determinater was controlled synergistically by both 2a and 2b encoded by RNA2 of 20.
Subjects
香蕉嵌紋病
胡瓜嵌紋病毒
banana mosaic
cucumber mosaic cucumovirus
symptom determinater
Type
other
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