Functional studies of Drought-Induced Protein 19-2 in flowering time and salt stress response in Arabidopsis thaliana
Date Issued
2015
Date
2015
Author(s)
Wu, Tien-Lun
Abstract
The Arabidopsis Drought-Induced Protein 19 (Di19) gene family encodes seven Cys/His-type zinc-finger proteins, most of which with unknown fuctions. The Di19s contain two atypical Cys/His zinc finger-like domains which may participate in different abiotic stress responses and signaling pathways. It was known that the transcripts of Di19-2 (At1G02750) increased in response to high-salt stress, but the protein expression pattern and functions remain unknown. Previous study showed that Di19-2 can interact with IDD8/NUC in yeast two-hybrid, pull-down and bimolecular fluorescence complementation (BiFC) assays. Here, it was found that Di19-2 interacts with IDD8/NUC and positively coregulate SUCROSE SYNTHASE 4 (SUS4) gene expression, but Di19-2 does not recognize Di19 binding site (DiBS, a known Di19-1/Di19-3 binding sequence). After generating the Di19-2 overexpression line, it was found the primary roots of Di19-2 overexprssion transgenic seedlings were shorter than wild type when encountered salt stress. Moreover, the adult of Di19-2 overexprssion transgenic plants showed lower salt and drought tolerance in the soil, which suggests that Di19-2 may play important role in salt and drought stress response. Interestingly, Di19-2 overexprssion transgenic plants showed early flowering phenotype. The flowering regulation genes were up-regulated in Di19-2 overexprssion transgenic plants. When idd8/nuc was crossed to Di19-2 overexprssion line, it showed the recovery of delay flowering phenotype in idd8/nuc mutant line. This suggests that Di19-2 may interact with IDD8/NUC to co-regulate flowering in Arabidopsis.
Subjects
Drought-induced protein 19
Di19 binding site (DiBS)
salt stress
drought stress
flowering
Type
thesis
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