The application of a duplex reverse transcription real-time PCR for the surveillance of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2
Journal
Journal of Virological Methods
Journal Volume
201
Pages
13-19
Date Issued
2014
Author(s)
Deng, Ming-Chung
Yang, Chia-Huei
Chang, Chieh
Huang, Yu-Liang
Abstract
The porcine respiratory disease complex (PRDC) is the most common disease in commercial pork production worldwide. Porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV), the most important agents of PRDC, usually co-infect in the same pigs. In order to survey the prevalence of PCV2 and PRRSV in pigs of various ages, a duplex reverse transcription real-time PCR (DRT-rPCR) was developed and applied in the present study. The DRT-rPCR did not cross-react with 10 swine viruses other than PCV2 and PRRSV, with detection limits of 1 TCID50/ml for PCV2 and 6.3 TCID50/ml for PRRSV. Surveillance using DRT-rPCR together with serology revealed that in the five farms studied, pigs were most susceptible to PRRSV at 6-14 weeks of age, whereas susceptibility to PCV2 varied by the management system but was mostly at 10-14 weeks of age. Cross analysis of viral loads versus antibody titers revealed that PCV2 load was affected negatively by anti-PCV2 ORF2 antibody, which constituted the most important non-infectious factor affecting the development of PMWS. These results indicated that DRT-rPCR was developed and applied successfully to the surveillance of PCV2 and PRRSV in the field. © 2014 Elsevier B.V.
Subjects
Duplicate reverse transcription real-time PCR; Porcine circovirus type 2; Porcine reproductive and respiratory syndrome virus; Surveillance
SDGs
Other Subjects
virus antibody; virus antibody; age; animal cell; animal tissue; antibody titer; Arterivirus; article; Circovirus; controlled study; diagnostic test accuracy study; disease surveillance; duplex reverse transcription real time polymerase chain reaction; infection sensitivity; intermethod comparison; limit of detection; nonhuman; open reading frame; pig farming; porcine circovirus type 2; porcine respiratory disease complex; prevalence; priority journal; real time polymerase chain reaction; reproducibility; sensitivity and specificity; serology; swine disease; virus detection; virus load; animal; Arterivirus; blood; Circoviridae Infections; Circovirus; epidemiological monitoring; evaluation study; genetics; isolation and purification; multiplex polymerase chain reaction; porcine reproductive and respiratory syndrome; procedures; real time polymerase chain reaction; reverse transcription polymerase chain reaction; swine; Swine Diseases; veterinary; virology; Animals; Antibodies, Viral; Circoviridae Infections; Circovirus; Epidemiological Monitoring; Multiplex Polymerase Chain Reaction; Porcine Reproductive and Respiratory Syndrome; Porcine respiratory and reproductive syndrome virus; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Sensitivity and Specificity; Swine; Swine Diseases; Viral Load
Type
journal article