Synthesis of Fluorescent Cerium/Gold Nanoclusters as pH Sensors
Date Issued
2014
Date
2014
Author(s)
Chen, Ya-Na
Abstract
Novel bovine serum albumin (BSA) templated cerium/gold nanoclusters have been prepared using a facile one-pot heating synthetic method. The as-prepared nanoclusters, when irradiated with 325 nm UV light, possess two fluorescence emission peaks at 410 nm and 650 nm. The emission maximum at 410 nm is attributed to the contribution of Ce4+ complexed with BSA, and the emission at 650 nm is in the characteristic emission range of BSA-Au NCs. The peak at 410 nm is found to be sensitive to the pH values of the environment, while the emission at 650 nm is pH-inert. Therefore, BSA-Ce/Au NCs with emission peaks located at 410 nm and 650 nm can serve as a ratiometric pH sensor, using the ratio I410 / I650. Using protein as the synthetic template, BSA-Ce/Au NCs are found to be highly biocompatible, and its fluorescence intensities are stable against salt and constant irradiation. Confocal images of HeLa cells have proven that this probe can be used for detection in biological systems, and that altering the environmental pH of the cells, BSA-Ce/Au NCs could reflect the resulting intracellular changes.
Subjects
鈰
金
奈米團簇
細胞影像
酸鹼感測器
Type
thesis
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