Applicability of Non-Invasive Sampling in Population Genetic Study of Taiwanese Macaques (Macaca cyclopis)
Resource
Taiwania, 51(4), 258-265
Journal
Taiwania
Journal Volume
51
Journal Issue
4
Pages
258-265
Date Issued
2006-12
Date
2006-12
Author(s)
Chu, J.H.
Lin, Y.S.
Wu, H.Y.
Abstract
This paper presents a pilot study conducted to test the applicability of non-invasive sampling approach in population genetic studies of Taiwanese macaques (Macaca cyclopis). Monkey feces were collected in the field and used as non-invasive DNA sources. PCR success rates of both microsatellite and mitochondrial DNA markers were examined. When compared with other studies by non-invasive genetic sampling of different mammal species, success rate of microsatellite PCR amplification is low (42.4%, N = 181) while that of mtDNA PCR amplification is acceptable (66.5%, N = 334). The low PCR success rate and poor PCR repeatability of microsatellite alleles due to allelic dropout and false alleles make it difficult to obtain a reliable microsatellite data set. However, the difficulties may be overcome by new techniques.
Subjects
Macaca cyclopis
Non-invasive sampling
Microsatellite
mtDNA d-loop
臺灣獼猴
非侵?性採樣法
微隨體
?線體DNA d-loop
Type
journal article
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