An Aligned 3D Collagen Matrix for the Study of Cell Migration
Date Issued
2011
Date
2011
Author(s)
Feng, Chia-Hsiang
Abstract
The aim of this study is to develop an aligned collagen matrix to examine cell migration of ligament fibroblasts, which natively reside in a highly collagen fibrous and anisotropic microenvironment made of type I collagen. This highly aligned arrangement has been shown to modulate fibroblast morphology, migration rate and attachment. However, cells also exhibit significantly different behaviors in 2D and 3D environments. In the current study, a microfluidic device was developed to create aligned collagen hydrogel and examine cell migration behavior in 2D and 3D collagen matrix. Cells are subjected with an electrical field to apply a homogenous field of migration stimulation.
Cells in both 2D and 3D show high alignment to the collagen matrix and cell migration show preference of contact guidance over EF stimulation. However, cell migration directionality is influenced by matrix thickness, possibly through regulation of cell attachment disassembly and tensional homeostasis.
Subjects
Cell migration
3D collagen matrix
tensional homeostasis
electrical field
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-100-R98548058-1.pdf
Size
23.54 KB
Format
Adobe PDF
Checksum
(MD5):312f38478921683a6e14207d17ed15e4