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  2. College of Bioresources and Agriculture / 生物資源暨農學院
  3. School of Veterinary Medicine / 獸醫專業學院
  4. Veterinary Medicine / 獸醫學系
  5. Systemic exposure to a single dose of ferucarbotran aggravates neuroinflammation in a murine model of experimental autoimmune encephalomyelitis
 
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Systemic exposure to a single dose of ferucarbotran aggravates neuroinflammation in a murine model of experimental autoimmune encephalomyelitis

Journal
International Journal of Nanomedicine
Journal Volume
14
Pages
1229-1240
Date Issued
2019
Author(s)
Hsiao Y.-P.
Huang C.-H.
Lin Y.-C.
TONG-RONG JAN  
DOI
10.2147/IJN.S189327
URI
https://scholars.lib.ntu.edu.tw/handle/123456789/444590
URL
https://www2.scopus.com/inward/record.uri?eid=2-s2.0-85062859287&doi=10.2147%2fIJN.S189327&partnerID=40&md5=1ba5e565917377f92c344a94f2f146b3
Abstract
Background: Medicinal preparations of iron oxide nanoparticles (IONPs) have been used as MRI contrast agents for the diagnosis of hepatic tumors and the assessment of neuroinflammation and blood–brain barrier integrity. However, it remains mostly unclear whether exposure to IONPs affects neuroinflammation under disease conditions. The present study aims to investigate the impact of IONPs on autoimmune-mediated neuroinflammation using a murine model of experimental autoimmune encephalomyelitis (EAE) that mimics human multiple sclerosis. Methods: Mice were either left untreated or immunized with myelin oligodendrocyte glyco-protein on day 0 followed by two injections of pertussis toxin for EAE induction. The EAE mice were intravenously administered with a single dose of the carboxydextran-coated IONPs, ferucarbotran (20 mg Fe/kg) and/or saline (as vehicle) on day 18. Symptoms of EAE were daily monitored until the mice were killed on day 30. Tissue sections of the brain and spinal cord were prepared for histopathological examinations. Iron deposition, neuron demyelination and inflammatory cell infiltration were examined using histochemical staining. The infiltration of microglial and T cells, and cytokine expression were examined by immunohistochemical staining and/or reverse transcription polymerase chain reaction (RT-PCR). Results: Iron deposition was detected in both the brain and spinal cord of EAE mice 3 days post-ferucarbotran treatment. The clinical and pathological scores of EAE, percentage of myelin loss and infiltration of inflammatory cells into the spinal cord were significantly deteriorated in EAE mice treated with ferucarbotran. Furthermore, ferucarbotran treatment increased the number of CD3 + , Iba-1 + , IL-6 + , Iba-1 + TNF-α + and CD3 + IFN-γ + cells in the spinal cord of EAE mice. Conclusion: A single exposure to ferucarbotran exacerbated neuroinflammation and disease severity of EAE, which might be attributed to the enhanced activation of microglia and T cells. These results demonstrated that the pro-inflammatory effect of ferucarbotran on the central nervous system is closely associated with the deterioration of autoimmunity. ? 2019 Hsiao et al.
Subjects
Experimental autoimmune encephalomyelitis; Ferucarbotran; Iron oxide nanoparticles; Microglia; Multiple sclerosis; Neuroinflammation; T cell
SDGs

[SDGs]SDG3

Other Subjects
calcium binding protein; CD3 antigen; cytokine; ferucarbotran; gamma interferon; interleukin 6; myelin; myelin oligodendrocyte glycoprotein; pertussis toxin; protein Iba 1; sodium chloride; tumor necrosis factor; ultrasmall superparamagnetic iron oxide; unclassified drug; cytokine; dextran; iron; magnetite nanoparticle; messenger RNA; superparamagnetic iron oxide; allergic encephalomyelitis; animal cell; animal experiment; animal model; animal tissue; Article; autoimmunity; brain tissue; cell activation; cell infiltration; central nervous system; controlled study; demyelination; disease association; disease severity; experimental autoimmune encephalomyelitis; experimental neuroinflammation; female; histochemistry; histopathology; immunohistochemistry; inflammatory cell; iron metabolism; microglia; mouse; multiple sclerosis; nervous system inflammation; nonhuman; pathophysiology; population exposure; protein depletion; protein expression; reverse transcription polymerase chain reaction; spinal cord; T lymphocyte; T lymphocyte activation; animal; C57BL mouse; disease model; experimental autoimmune encephalomyelitis; genetics; human; immunology; inflammation; metabolism; pathology; severity of illness index; Animals; Central Nervous System; Cytokines; Dextrans; Disease Models, Animal; Encephalomyelitis, Autoimmune, Experimental; Female; Humans; Inflammation; Iron; Magnetite Nanoparticles; Mice, Inbred C57BL; RNA, Messenger; Severity of Illness Index; Spinal Cord; T-Lymphocytes
Type
journal article

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