Reishi protein LZ-8 induces FOXP3+ treg expansion via a CD45-dependent signaling pathway and alleviates acute intestinal inflammation in mice
Journal
Evidence-based Complementary and Alternative Medicine
Journal Volume
2013
Date Issued
2013
Author(s)
Abstract
LZ-8, an immunomodulatory protein isolated from Ganoderma lucidum (also known as Ling-Zhi or Reishi), has been shown to promote cell proliferation and IL-2 production in T cells. In this study, we show that LZ-8 induces the expansion of both murine and human CD4+ T cells into FOXP3 + regulatory T (Treg) cells. LZ-8 treatment was found to stimulate a 4-fold and a 10-fold expansion in the Treg populations of murine and human primary CD4+ T cells, respectively. In addition, the expression of CTLA-4 and IL-10 was induced in LZ-8-treated CD4+ T cells. Using neutralizing antibodies and gene-deficient T-cell lines, we also found that LZ-8 promotes Treg expansion through a CD45-mediated signaling pathway and that the CD18-dependent induction of IL-2 was involved in Treg formation and IL-10 production. The suppressive activity of LZ-8 was confirmed using a murine model of DSS-induced colitis; the disease was alleviated by the adoptive transfer of LZ-8-treated CD4+ T cells. In conclusion, a new regulatory function for LZ-8 was identified, and the molecular mechanisms underlying this function were elucidated. ? 2013 Hsien-Yeh Hsu et al.
SDGs
Other Subjects
CD18 antigen; CD45 antigen; cytotoxic T lymphocyte antigen 4; fungal protein; interleukin 10; interleukin 2; LZ 8 protein; transcription factor FOXP3; unclassified drug; adoptive transfer; animal cell; animal experiment; animal model; article; CD4+ T lymphocyte; cell expansion; controlled study; cytokine production; enteritis; human; human cell; male; mouse; nonhuman; priority journal; protein expression; protein function; protein induction; regulatory T lymphocyte; signal transduction
Type
journal article