The Role of Nuclear CTEN in Tumorigenesis
Date Issued
2015
Date
2015
Author(s)
Huang, Yen-kang
Abstract
C-terminal tensin-like (CTEN) locates in focal adhesion and belongs to tensin family. CTEN expresses much in prostate and placenta, but rarely expresses in other normal tissues. Elevated CTEN level has been detected in lung cancer, colorectal cancer and breast cancer. In addition to focal adhesion, CTEN also accumulates in the nucleus in colorectal cancer cell lines SW480 and HCT 116. It has been found that CTEN in nucleus interacts with Wnt pathway signal beta-catenin and promotes tumorigenesis. On the other hand, human kidney embryonic cell 293A and normal prostate cell RWPE-1 express CTEN predominantly in cytoplasm. It is shown that CTEN localization is related to tumorigenesis. We assumpted that CTEN accumulation in the nucleus will lead to tumor. By adding special signaling amino acid sequence, nucleus localization signal (NLS) and Src myristoylation signal cause CTEN to change localization in cell. Therefore, we can observe tumorigenesis of cells with different CTEN localization. First, we established stable cell lines expressing CTEN protein with special amino acid signals. We checked with western blot and immunofluorescence, and we confirmed that expressing CTEN with different signals does cause CTEN distribution changes. Then, we used cell proliferation assay to observe the effect of CTEN distribution in tumorigenesis, and we found that CTEN accumulation in cell nucleus do not affect cell proliferation rate.
Subjects
Tumorigenesis
CTEN
tensin
distribution
localization
cancer
tumor
membrane-targeting
nucleus-targeting
cell proliferation rate
SDGs
Type
thesis