Isolation and Identification of Human Metapneumovirus in Taiwan
Date Issued
2005
Date
2005
Author(s)
Chen, Bao-Chen
DOI
zh-TW
Abstract
Human metapneumovirus (HMPV) was first identified in 2001 in The Netherlands from infants and children with acute respiratory tract disease and is now recognized to be worldwide in prevalence. This is the first report that human Metapneumovirus (hMPV) were isolated from clinical specimens and associated with respiratory tract disease in Taiwan.
The hMPV were screened by culture in LLC MK2 cell from 4726 respiratory specimens collected from Dec. 2003 to April 2005 in Taiwan. Eight (0.17%) hMPV strains were isolated and comparison of aligned F gene and N gene sequence were divided into 2 subtype A1, 4 subtype B1, and one each for A2 and B2 subtypes.
Our own observations indicate that hMPV were 2 different morphologies of CPE in growth LLC MK2, consisting of rounded cells, focal plaques and with cell fusion. The CPE was apparent after a mean incubation time of 20 days (15–25days). The key element of observation of CPE is pH of the medium. When hMPV culture in pH 7.0 and 7.5 medium, we can obtain obvious CPE. In search of a cell line more susceptible for hMPV, we generated 7 subclones of LLC MK2 cell, CPE produced more prominent in LLC MK2 subclones (3 days), compared to LLC MK2 (10 days) when culture in pH7.0 and 7.5 medium.
Serology tests were performed to investigate the prevalence of hMPV in Taiwan. The 116 serum samples from children aged from less one-week new bone babies to 84 years adults were tested for the presence of hMPV antibody using an indirect immunofluorescence assay. Preliminary result indicated that all baby (less than 0.5 years old) had hMPV antibody. After than, the seropositive rate decreased to 60% in children from 0.5 to 1.5 years old. The seropositive rate increased gradually to 100% from adolescent to adult (≦40 years old). Further prospective studies are required to define the epidemiology and the extent of hMPV infection in Taiwan.
Subjects
間質性肺炎病毒
Metapneumovirus
SDGs
Type
other
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