Studies of Transposable Element Tag1 and miRNA Formation
Date Issued
2013
Date
2013
Author(s)
Lu, Hsin-Chieh
Abstract
It has been proved that there is a close association between miRNA and transposable element (TE). Some TE may form a secondary structure which is similar to the hairpin structure of miRNA. In this study, we use an intrinsic TE, Tag1, from Arabidopsis thaliana Landsberg erecta ecotype. Tag1 is 3.3 kb in length and has 22-bp terminal inverted repeat ( TIR ) which can perfectly match. We questioned that whether these sequences can form a hairpin structure at RNA level, and then control the target gene expression by miRNA formation. PCR technique was employed to amplify the 5’ and 3’ subterminal fragments ( 270 and 262 bp ) of Tag1 sequence to yield the mini Tag (532 bp). To analyze the expression, the mini Tag was inserted into the 5’ UTR and 3’ UTR of a reporter gene luc, and was transformed into Nicotiana tabacum by Agrobacterium tumefaciens. The results of in vivo luciferase assay show that all the transformants have the luciferase activity. This outcome indicates that the mini Tag element may not form an effective hairpin structure or the formation of secondary structure occurs but it cannot induce to RNA interference pathway. Finally, we discuss the correlation between the TE and miRNA, and introduce a new tool of gene regulation.
Subjects
轉位子
雙股RNA
阿拉伯芥
基因靜默
演化
Type
thesis
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