Application of metabolomics approach to find biomarkers and mechanisms of turmeric effect on working memory in pre-diabetes
Date Issued
2016
Date
2016
Author(s)
Wu, Feng-Ju
Abstract
Background and objective: Our previous study showed that the intake of turmeric with white bread could acutely increase working memory in patient with prediabetes. However, the effect was not associated with known AD biomarkers, more research is needed to understand the potential mechanisms. Metabolomics is a systematic platform which appraises all small molecule metabolites in a bio-specimen. Exploring metabolomics changes may help us to find new biomarkers and mechanisms associating with working memory. This study was a double-blind randomized placebo controlled metabolics study. We intended to use analytical metabolomics approach to find specific metabolic changes associated with turmeric intervention. Method: The study included 2 groups: the experimental group (n=12) provided with 1g of turmeric capsule and with two slices of bread, whereas the control group (n=11) provided with placebo. The observation period lasted for 6 hours collecting the plasma samples at four time points and appraising working memory (WM) before and after the 6 hr period. We applied a comprehensive UPLC-MS platform to carry out untargeted plasma metabolomics. Using generalized estimating equation (GEE) models, we carried out statistical analyses to compare turmeric groups with placebo group through time points on mean intensity difference and on slope of intensity changes. Results: Metabolite markers identified were involved in carbohydrate metabolism, amino acid metabolism, nucleotide metabolism, lipid metabolism, etc. The changes (AUC) of S-adenosyl-L-homocysteine was inversely associated with WM changes; whereas trans-cinnamic acid was found positively associated with WM changes. These findings provide a comprehensive global plasma metabolite profiling and may contribute to understanding the pathogenic mechanism of how turmeric improved WM performance. Conclusion: In conclusion, we identified comprehensive metabolic shifts between turmeric and placebo subjects including altered carbohydrate metabolism, nucleotide metabolism, lipid metabolism, amino acid metabolism, etc. The WM improved by turmeric administration may on account of changes in cysteine and adenosine metabolism as well as phenylalanine metabolism identified in our study. This study helps us approach the potential mechanism of turmeric effect on working memory.
Subjects
metabolomics
plasma
turmeric
working memory
SDGs
Type
thesis
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