Phosphoproteome of cRGD-induced MCF-7 cell death
Date Issued
2007
Date
2007
Author(s)
Shih, Ching-Yao
DOI
zh-TW
Abstract
The RGD (arginine-glycine-aspartate) motif is an integrin-recognition motif found in many ligands, so that RGD-containing peptides can interact with the integrin receptor to induce cell-signaling processes and influence many different diseases. Synthetic peptides containing the RGD motif have been used extensively as inhibitors of integrin-ligand interactions in studies of cell growth, death, adhesion, migration, and invasion. Therefore, we designed a cyclic-RGD peptide (Tpa-RGDWPC, cRGD) with rigid skeleton to closely bind with its receptor. According to previous reports that cRGD can cause cell death in MCF-7 breast cancer cell line. We are interested in what molecular mechanism underlies the cRGD exerts on MCF-7 cells. Depending on protein phosphorylation, it is a major post-translational modification connected to the signaling pathways in the cell. We used the proteomics with Pro-Q Diamond phosphoprotein dye technology to survey the global changes in phosphoproteins after cRGD treatment in MCF-7 cells. In this report, we find some phosphoproteins play important roles in cRGD treated MCF-7 cell death and provide a valuable in-depth insight into its use in breast cancer therapy.
Subjects
整合素
RGD
乳癌
細胞死亡
磷酸化蛋白
Integrin
Breast cancer
Cell death
Phosphoprotein
SDGs
Type
other
File(s)![Thumbnail Image]()
Loading...
Name
ntu-96-R94B43025-1.pdf
Size
23.31 KB
Format
Adobe PDF
Checksum
(MD5):39e7c1b88ccc8fb016ae2f06d82db288