Association of Nak with AP2 in Endocytosis
Date Issued
2007
Date
2007
Author(s)
Lai, Tzu-Ting
DOI
en-US
Abstract
Endocytosis is a fundamental step to regulate a diverse array of cellular activities. In response to signals, the process of endocytosis can mediate membrane receptor recycling, and cargos delivery to intracellular organelles. The phosphorylation state of endocytic components, such as the adaptor protein complex 2 (AP2) and the engulfed membrane, can modulate clathrin-coated pit formation and cargo selection. The ARK family of serine/threonine kinases is shown to regulate endocytosis through the phosphorylation of various endocytic components. Numb associated kinase (Nak), the only ARK member in Drosophila, bears a serine/threonine kinase domain at the amino-terminus and various endocytic motifs, including DLL for interacting with clathrin and DPF for α-adaptin. To investigate possible signaling pathways and cellular mechanisms regulated by Nak, I have performed immunoprecipitation for Nak and identified proteins associated with Nak by Mass-Spectrometry (MS). Through the MS analysis, I have identified α-adaptin, β-adaptin and the u2 subunit AP50 of the AP2 complex. With the complex association in the dynamin mutant, shibire, I suggest that Nak may take part in late steps of endocytosis. I also performed a male recombination experiment to screen for nak mutants for further functional analysis. Several hypomorphic alleles obtained in this screening show reductions in mRNA and protein levels.
Subjects
內吞作用,Nak,AP2
endocytosis,Nak,AP2
Type
other