Attractable ability of light, color and temperature in dengue vector mosquitoes and their gene expressions of heat-activated channel TRPA1
Date Issued
2014
Date
2014
Author(s)
Tsai, Chia-Hung
Abstract
Dengue fever is one of the most serious insect-borne infectious diseases in Taiwan. It has no vaccine or medicine for dengue now, so it should be relying on vector control. This study is aimed to develop physical strategies for trapping Aedes aegypti and Ae. albopictus by potential attractable factors, including light, color and temperature, as well as to analyze the performance of heat-activated channel gene expression. The results obtained from our laboratory experiment showed that ultraviolet light (360 nm) was more effective to attract diurnal Ae. mosquitoes than blue (450 nm), green (510 nm) and red (630 nm) light be. The attraction rate of female Ae. aegypti (n=50) and male (n=50) were 42.00±7.94% and 10.00±6.24%, and female Ae. albopictus (n=50) and male (n=50) were 46.33±3.78% and 15.67±4.04%. The preferences of female Ae. aegypti (n=25) to colors was shown in the following: black (24.53±2.02%) > deep blue (21.87±1.52%) > red (14.13±1.19%) > green (6.67±2.11%) > light blue (4.53±1.79%) > yellow (1.33±0.00%). Similarly, female Ae. albopictus to colors was: red (25.6±2.89%) > black (23.73±1.16%) > deep blue (20.53±0.73%) > light blue (6.4±1.16%) > green (5.6±1.11%) > yellow (2.4±2.89%). The combination of black color and ultraviolet for light trap led to higher attraction rate (62.42±8.03%) toward Ae.aegypti, and rate (76.05±10.34%) toward Ae. albopictus. Temperatures ranging from 26 ° C to 38 ° C could attract both Ae. aegypti and Ae. albopictus. The attraction rates of female Ae. aegypti (n=25) and Ae. albopictus (n=25) were 45.14±3.02% and 40.57±6.70%, respectively. The condition of color paper covered on heater at 32 °C brought higher attraction rate: black (70.67±4.62%) > deep blue (65.33±8.33%) > red (50.67±9.24%) > uncovered (45.33±10.07%) > white (38.67±8.33%). Similarly, female Ae. albopictus was：black (70.67±8.32%) > deep blue (72.00±6.92%) > red (65.33±8.32%) > uncovered (48.00±6.92%) > white (41.22±6.11%). In the studies on Drosophila, TRPA was affected by ambient temperature. In other studies, it was found that TRPA1 on Anopheles gambiae antennae acted as a kind of channels which would be excited by heat. Therefore, not only do we want to figure out attraction effect of heat on mosquitoes, but to understand levels of heat-induced gene expression in Ae. aegypti and Ae. albopictus. So this study separated the heads from mosquitoes, which were subsequently sorted into heat-attractable and non-attractable group. Then the total RNA was extracted from the heads followed by TRPA1 gene amplification using RT-PCR. The results showed that the level of TRPA1 expression in Ae. aegypti heads of attractable group were 1.48 times higher than non-attractable group, and those in heads of Ae. albopictus was 1.59 times as much as non-attractable group. Based on the these results, we got a specific wavelength, color and temperature that are able to efficiently attract Ae. aegypti and Ae. albopictus. And TRPA1 gene was found to be activated with heat excitement in Ae. aegypti. In the future work, based on our findings, we hope to develop a novel mosquito trap with more effective capacity for diurnal mosquitoes control, which could be expected to be applied in practical control of mosquitoes and prevention of dengue fever.
Subjects
登革熱
病媒蚊防治
波長
顏色
溫度
TRPA1
SDGs
Type
thesis
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