Imaging and Analysis of Single Skin Cell by Ultrahigh-Resolution Optical Coherence Tomography
Date Issued
2012
Date
2012
Author(s)
Hsieh, Tsung-Hsun
Abstract
Ultrahigh-resolution optical coherence tomography (UR-OCT) has been used to study single skin cells in vitro for the first time. This non-invasive and label-free optical detection technique with deep imaging depth can be used to analyze scattering properties of single cells in three-dimensional with cellular spatial resolution. Based on morphological observation of the UR-OCT images, live and apoptotic basal cell carcinoma (BCC) can be easily identified. In addition, we developed a novel method to automatically extract the characteristic parameters which defined as signal average, cell volume, cellular density and average dynamic range of individual cells. Quantitative comparison showed that three parameters were found with p-value smaller than 0.05. The experimental results show that the ability of UR-OCT to detect cell death at cellular level.
To find the nuclear-cytoplasmic ratio (N:C ratio) of single cells, we did a series of image segmentation processes by means of morphological dilation and erosion. In this way, calculated N:C ratio of single HaCaT cell was 0.18, which was very close to the observation of confocal fluorescence microscopy.
Finally, the HaCaT and Hs68 cell lines were used to model epidermis and dermis respectively. We applied clustering analysis on these cells by k-means clustering method based on the characteristic parameters. We demonstrate that a total of 12 HaCaT and Hs68 cells can be distinguished very quickly and accurately, indicating that the UR-OCT will play an important role in clinical dermatology and tissue engineering in the future.
Subjects
Optical coherence tomography
Single cell
Basal cell carcinoma
Apoptosis
Type
thesis
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