Assembly of Interfacial Polyelectrolyte Complexation Fibers with Mineralization Gradient for Physiologically-Inspired Ligament Regeneration.
Journal
Advanced materials (Deerfield Beach, Fla.)
Series/Report No.
Advanced Materials
Journal Volume
36
Journal Issue
25
ISSN
1521-4095
Date Issued
2024-06
Author(s)
Liu, Yu-Chung
Chen, Shih-Heng
Chen, Shih-Hsien
Huang, Wei-Yuan
Chen, Hao-Xuan
Wang, Tzu-Wei
DOI
10.1002/adma.202314294
Abstract
Current synthetic grafts for ligament rupture repair often fail to integrate well with the surrounding biological tissue, leading to complications such as graft wear, fatigue, and subsequent re-rupture. To address this medical challenge, this study aims at advancing the development of a biological ligament through the integration of physiologically-inspired principles and tissue engineering strategies. In this study, interfacial polyelectrolyte complexation (IPC) spinning technique, along with a custom-designed collection system, to fabricate a hierarchical scaffold mimicking native ligament structure, is utilized. To emulate the bone-ligament interface and alleviate stress concentration, a hydroxyapatite (HAp) mineral gradient is strategically introduced near both ends of the scaffold to enhance interface integration and diminish the risk of avulsion rupture. Biomimetic viscoelasticity is successfully displayed to provide similar mechanical support to native ligamentous tissue under physiological conditions. By introducing the connective tissue growth factor (CTGF) and conducting mesenchymal stem cells transplantation, the regenerative potential of the synthetic ligament is significantly amplified. This pioneering study offers a multifaceted solution combining biomimetic materials, regenerative therapies, and advanced techniques to potentially transform ligament rupture treatment.
Subjects
bone‐ligament interface
hierarchical structure
interfacial polyelectrolyte complexation
ligament tissue engineering
mineralization gradient
Publisher
Wiley
Type
journal article