Diverse effects of phytoestrogen biochanin A on rat pituitary tumor cells
Journal
Journal of Food and Drug Analysis
Journal Volume
12
Journal Issue
1
Pages
7-12
Date Issued
2004
Author(s)
Abstract
Biochanin A, a nonsteroidal estrogen present in plants, has been suggested in epidemiological studies to be associated with a reduced risk of hormone-related cancers. In this study, we examined the effects of biochanin A on the cell growth and gene expression of an estrogen-responsive pituitary cell line GH3. Results showed that biochanin A inhibited growth of GH3 cells in a dose-dependent manner and at physiologically relevant concentrations. At concentrations of 1 and 5 μg/mL, inhibition of cell growth was observed. The gene expressions of estrogen receptor (ER) mRNA and an estrogen responsive gene, the progesterone receptor (PR), were down-regulated by the biochanin A treatment. In addition, co-administration of antiestrogen ICI182,780 blocked this biochanin A induced down-regulation of the ER and PR mRNA level. However, the expression level of rat prolactin (rPRL) gene, one of the typical estrogen responsive genes, was not regulated by biochanin A. These results indicated that biochanin A at physiologically achieveable concentrations was able to inhibit cell growth of estrogen-responsive rat pituitary cells. In addition, dietary-derived biochanin A compounds exhibit diverse estrogenic and antiestrogenic effects on estrogen-related gene expression, suggesting gene-specific regulation.
Subjects
Cell growth; Dietary estrogen; Gene expression; Pituitary cells
SDGs
Other Subjects
biochanin A; estrogen receptor; messenger RNA; phytoestrogen; progesterone receptor; prolactin; animal cell; article; cancer inhibition; cell culture; cell growth; cell proliferation; cell viability; concentration (parameters); concentration response; controlled study; drug mechanism; gene control; gene expression; growth inhibition; hypophysis tumor; immunoprecipitation; nonhuman; rat; receptor down regulation; reverse transcription polymerase chain reaction; statistical analysis; statistical significance; tumor cell line; Western blotting; Animalia
Type
journal article