The regulatory role of hnRNP K phosphorylation to COX-2 mRNA stability in mouse macrophage RAW264.7 cells
Date Issued
2012
Date
2012
Author(s)
Chiang, Pei-Yu
Abstract
When pathogen infection occurrs in organism, many cytokines or immediate genes (IEGs) are rapidly and precisely expressed and/or repressed for performing immune responses. Tight control of these genes is very important for preventing immune disorders. Post-transcriptional regulation involving in trans-acting RNA-binding proteins is one of the favorite strategies of cells to manipulate gene expression. Heterogeneous nuclear ribonucleoprotein K (hnRNP K), containing three KH (K-homology) domains that mediate RNA binding, is involved in the regulation of post-transcription and translation processes. To study the possible functions of hnRNP K in immune response, I performed anti-hnRNP K RNA immunoprecipitation assay with cell extracts from lipopolysaccharide (LPS)-treated mouse RAW264.7 macrophages. Two IEGs, Tistetraproline (TTP) and Tumor necrosis factor
Subjects
LPS, macrophage, RAW264.7, hnRNP K, COX-2, post-transcriptional regulation
Type
thesis
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