MBP-1 suppresses growth and metastasis of gastric cancer cells through COX-2
Journal
Molecular Biology of the Cell
Journal Volume
20
Journal Issue
24
Pages
5127-5137
Date Issued
2009
Abstract
The c-Myc promoter binding protein 1 (MBP-1) is a transcriptional suppressor of c-myc expression and involved in control of tumorigenesis. Gastric cancer is one of the most frequent neoplasms and lethal malignancies worldwide. So far, the regulatory mechanism of its aggressiveness has not been clearly characterized. Here we studied roles of MBP-1 in gastric cancer progression. We found that cell proliferation was inhibited by MBP-1 overexpression in human stomach adenocarcinoma SC-M1 cells. Colony formation, migration, and invasion abilities of SC-M1 cells were suppressed by MBP-1 overexpression but promoted by MBP-1 knockdown. Furthermore, the xenografted tumor growth of SC-M1 cells was suppressed by MBP-1 overexpression. Metastasis in lungs of mice was inhibited by MBP-1 after tail vein injection with SC-M1 cells. MBP-1 also suppressed epithelial-mesenchymal transition in SC-M1 cells. Additionally, MBP-1 bound on cyclooxygenase 2 (COX-2) promoter and downregulated COX-2 expression. The MBP-1-suppressed tumor progression in SC-M1 cells were through inhibition of COX-2 expression. MBP-1 also exerted a suppressive effect on tumor progression of other gastric cancer cells such as AGS and NUGC-3 cells. Taken together, these results suggest that MBP-1-suppressed COX-2 expression plays an important role in the inhibition of growth and progression of gastric cancer. ? 2009 by The American Society for Cell Biology.
SDGs
Other Subjects
cyclooxygenase 2; transcription factor; transcription factor mbp1; unclassified drug; article; cancer growth; cancer inhibition; cancer invasion; cell proliferation; colony formation; controlled study; down regulation; epithelium cell; gene expression; human; human cell; lung metastasis; mesenchyme; priority journal; promoter region; stomach adenocarcinoma; tumor xenograft; Western blotting; Amino Acids; Cell Line, Tumor; Cell Proliferation; Cyclooxygenase 2; Disease Progression; DNA-Binding Proteins; Down-Regulation; Epithelium; Gene Expression Regulation, Neoplastic; Humans; Mesoderm; Neoplasm Metastasis; Promoter Regions, Genetic; Protein Binding; Stomach Neoplasms; Structure-Activity Relationship; Mus
Type
journal article