離乳仔豬併欄時以Azaperone處理對血漿皮質醇與下視丘－腦下垂體－腎上腺軸緊迫相關基因表現變化之影響
Other Title
Effect of Azaperone on Plasma Cortisol and Stress-related Gene Expression in the Hypothalamus-Pituitary-Adrenal Axis in Co-mingling Weaned Pigs
Journal
臺灣獸醫學雜誌
Journal Volume
34
Journal Issue
3
Start Page
177
End Page
183
Date Issued
2008-09
Date
2008-09
Author(s)
Abstract
本試驗目的在探討抗緊迫藥物 azaperone 對離乳後併欄仔豬下視丘-腦下垂體-腎上腺軸 (Hypothalamus-pituitary-adrenal axis, HPA axis) 的影響。選用 18 頭 28 日齡來自不同 6 胎之仔豬，每胎 3 頭，逢機分配至 3 組：緊迫組 (S 組 ) 直接併欄，藥物處理組 (T 組 ) 於併欄時注射 azaperone，以及對照組 (C 組) 留在分娩舍。所有仔豬於併欄前及 2 小時後採血分析皮質醇 (cortisol) 值。第二次採血後將仔豬以電昏後放血犧牲，迅速取出下視丘、腦下垂體、腎上腺等組織置入液態氮中，並以即時反轉錄聚合鏈反應 (real-time RT PCR) 分別測定下視丘 c-fos 及 CRH、腦下垂體 c-jun 及 CRHR1、以及腎上腺 CRHR1 及 MC2R 等基因表現。結果顯示，併欄前後之血漿皮質醇值僅在 S 組有升高達顯著水準 (P < 0.05)。各基因表現以 C 組為基準進行比較，結果為 S 組及 T 組下視丘 c-fos 向下調控 (downregulation)，而腦下垂體 CRHR1 則呈向上調控 (up-regulation)，而 S 組與 T 組間各基因表現均無顯著差異。由以上結果得知離乳後併欄對仔豬會造成緊迫及 HPA 軸中下視丘、腦下垂體活性改變，而注射 azaperone 可以減緩緊迫但不影響 HPA 活性。[游建和、蕭世烜、陳靜宜、*張志成。離乳仔豬併欄時以 Azaperone 處理對血漿皮質醇與下視丘腦下垂體-腎上腺軸緊迫相關基因表現變化之影響。
This study was conducted to investigate the effect of azaperone on the plasma cortisol and stress-related gene expression in the hypothalamus-pituitary-adrenal (HPA) axis alteration in co-mingling weaned pigs. Eighteen 28 days old piglets from 6 litters were allocated to 3 groups, S: co-mingled group, T: azaperone treatment group and C: piglets stayed beside their dams in the far- rowing house. Blood samples were collected prior and 2 hours after co-mingling to determine the plasma cortisol change. After the second blood sampling, all animals were electrically euthanized and the HPA tissues were quickly removed for target gene mRNA quantification by real-time RT-PCR. The results showed that the concentrations of cortisol in S group are significantly higher (P＜0.05) than those in other groups. The gene expressions of hypothalamic c-fos in S and T group are down- regulated, and expressions of pituitary CRHR1 in S and T groups were up-regulated when compared to that in the C group. No significant differences on all these genes between S and T group were found. Thus, the co-mingling process was stressful to the weaned pigs, and azaperone may reduce the stress without influencing the stress-related gene expressions in the HPA axis.
Subjects
併欄
下視丘－腦下垂體－賢上腺軸
azaperone
co-mingling
HPA axis
Type
journal article
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