Hepatoprotective Activity of Ethnomedicinal Plants in Taiwan
Date Issued
2005
Date
2005
Author(s)
Yeh, Pei-Yun
DOI
zh-TW
Abstract
The aim of this study was to investigate the hepatoprotective activities, defined as capabilities against lipid peroxidation in mice, of 70% ethanol extracts of sixty-four ethnomedicinal plants in Taiwan. The anti-lipid peroxidative effects of plant extracts were determined by TBARS (thiobarbituric acid reactive substances) assay, which is an indicator of lipid peroxidation and free radical activity in an in vitro biological system. To induce the formation of lipid peroxidation the liver homogenates of mice were treated with tert-Butyl hydroperoxide (t-BuOOH) or ferrous chloride (FeCl2). The quantities of Malondialdehyde (MDA) thus induced were measured, after transforming into MDA (TBA) 2, at 532 nm by high performance liquid chromatography.
Compared to trolox, twenty-seven plant extracts (200μg/ml) prepared from leaf、stem or herb displayed significant inhibition (> 50%) on the formation of MDA: Rhus javanica (Anacardiaceae), Canna indica (Cannaceae), Bischofia javanica, Breynia officinalis, Mallotus paniculatus, Triadica sebifera (Euphorbiaceae), Cinnamomum camphora (Lauraceae), Desmodium sequax, Millettia pachycarpa, Pueraria montana (Leguminosae), Dianella ensifolia (Liliaceae), Ficus microcarpa (Moraceae), Psidium guajava (Myrtaceae), Areca catechu (Palmae), Rumex crispus (Polygonaceae), Spiraea prunifolia (Rosaceae), Psychotria rubra (Rubiaceae), Euphoria longana (Sapindaceae), Boehmeria densiflora, Boehmeria nivea (Urticaceae), and Tetrastigma formosanum (Vitaceae). The human normal liver cell (Chang liver cell lines) was used to test the cytotoxicity of the plant extracts. Cell activity were determined by the IC50-value of the reduced form of MTT (3-(4,5-dimethylthiazol)-2,5- diphenyltetrazolium bromide). Among these twenty-seven plant extracts (200μg/ml), only six exhibited no cytotoxicity: P. rubra, C. indica, B. javanica, B. officinalis, B. nivea, and T. formosanum. Furthermore, we evaluated the antioxidant activity of these six plant extracts by DPPH assay, metal (Fe2+) chelators, superoxide quencher, and FRAP assay. The results showed that these six plant extracts could scavenge DPPH free radical, in the order as following (IC50: μg/ml): P. rubra (5.54) > T. formosanum (13.77) > C. indica (19.02) > B. officinalis (20.78) > B. javanica (32.91) > B. nivea (43.91). They also exhibited ferric reducing /antioxidant power (equivalent μg/ml Trolox): P. rubra (24.24) > B. officinalis (11.56) > T. formosanum (10.59) > C. indica (9.37) > B. javanica (8.9) > B. nivea (6.74). But to our surprise these extracts showed no ability of chelating Fe2+ and scavenging superoxide free radical.
In conclusion, among sixty-four ethnomedicinal species thus studied, there are six plant extracts which demonstrate potential for protecting the mice liver from damage as caused by FeCl2, and which do not have cytotoxicity against Chang liver cell line. Moreover, the antioxidant activity of these six extracts is mainly mediated by theirfunction as hydrogen donators and reducing agents against the liver damage.
Subjects
總酚類含量
ICR鼷鼠
保肝
氯化亞鐵
脂質過氧化
細胞毒性
抗氧化能力
Ethnomedicinal plants
total polyphenol
ICR mice
ferrous chloride (FeCl2)
hepatoprotective
t-BuOOH
lipid peroxidation
cytotoxicity
antioxidant ability
Type
thesis
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