Enterovirus type 71 neutralizing antibodies in the serum of macaque monkeys immunized with EV71 virus-like particles
Journal
Vaccine
Journal Volume
30
Journal Issue
7
Pages
1305-1312
Date Issued
2012
Author(s)
Abstract
Enterovirus type 71 (EV71) is a virulent form of enteroviruses causing hospitalizations for children less than three years of age. Currently there are no anti-viral therapies or vaccines available for EV71. Due to the high risk of poliomyelitis-like paralysis and fatal encephalitis, an effective vaccine to EV71 could potentially prevent virus-induced morbidity and mortality. In this study, we first tested a potential EV71 vaccine candidate based on virus-like particles (VLP). We vaccinated macaque monkeys to validate the immunogenicity of the VLP vaccine to EV71. We detected the VLP or EV71-specific antibodies, neutralization titers, ELISPOT, and T cell response to find their immune responses to EV71. When the VLP vaccine adjuvanted with alum was given to macaque monkeys, these monkeys developed both specific humoral and cellular immune responses to EV71. Despite lower neutralizing antibodies to EV71 were found in sera of VLP-immunized monkeys than monkeys vaccinated with inactivated EV71, VLP-based vaccine generated a memory immune response to EV71. Hence, VLP-based EV71 vaccine is a potential vaccine against EV71 infection. ? 2012 Elsevier Ltd.
Subjects
Enterovirus; Immunity; Vaccine; Virus-like particle
SDGs
Other Subjects
aluminum hydroxide; Enterovirus 71 vaccine; neutralizing antibody; unclassified drug; virus like particle vaccine; article; cellular immunity; controlled study; Enterovirus 71; Enterovirus infection; enzyme linked immunospot assay; humoral immunity; immunogenicity; Macaca; nonhuman; priority journal; T lymphocyte; virion; Adjuvants, Immunologic; Animals; Antibodies, Neutralizing; Antibodies, Viral; Child, Preschool; Enterovirus A, Human; Enterovirus Infections; Humans; Immunity, Cellular; Immunity, Humoral; Immunologic Memory; Macaca; Vaccination; Vaccines, Inactivated; Vaccines, Virus-Like Particle; Viral Vaccines
Type
journal article