Protein-protein interaction between Arabidopsis drought-inducible Di19 protein and 14-3-3 protein
Date Issued
2009
Date
2009
Author(s)
Wang, Shu-Ying
Abstract
The Arabidopsis Di19 （Dehydration-induced protein 19） gene family encodes seven hydrophilic proteins, AtDi19-1~7, which contain two atypical Cys2/His2 zinc finger-like domains. It’s known that AtDi19 proteins can be involved in stress response and light signal transduction. AtDi19 proteins can be phosphorylated by CDPKs, but the function is not clear. Based on the results of protein sequence analysis, we identified putative 14-3-3 proteins binding motif in AtDi19-1, AtDi19-2 and AtDi19-5. 14-3-3 proteins are phosphoserine-binding proteins in eukaryotic cell, and may play an important role in responses to mineral nutrient deficiencies, ion transport and salt stress. We suspected that AtDi19 proteins may interact with 14-3-3 proteins. To investigate whether AtDi19 proteins can interact with 14-3-3 proteins, a yeast two-hybrid study was conducted. The result indicates that 14-3-3 omega can interact with AtDi19-1, 2 & 5.We also discovered that AtDi19-2-Arg98-His135 is the nessary region involved in the interaction with 14-3-3 omega. In addition, we tested if AtCDPK is involved in the interaction between 14-3-3 omega and AtDi19-2 by in vitro binding assay. According our results, AtCDPK is not involved in the interaction between Di19-2 and 14-3-3 omega.
Subjects
yeast two-hybrid, Di19 protein, 14-3-3 protein, protein-protein interaction
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