Characterization of copper/zinc and manganese superoxide dismutase in green bamboo (Bambusa oldhamii): Cloning, expression and regulation
Resource
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 49(2), 195-200
Journal
Plant Physiology and Biochemistry
Journal Volume
49
Journal Issue
2
Pages
195-200
Date Issued
2011
Date
2011
Author(s)
Abstract
Bamboo is distinguished by its rapid growth, for growth more than 100 cm per day. Because of the rapid growth, tissues have significant ATP requirements, which results in intense reduction of oxygen and thus oxidative stress. For this reason, bamboo may have a special and efficient scavenger system to release the stress during fast cell division and elongation. Here, we investigated superoxide dismutase (SOD, E.C.1.15.1.1), the first line of antioxidant enzymes, in green bamboo (Bambusa oldhamii). The SOD activity profile in this species was complex, with 5 genes and 7 isozymes of CuZnSOD and 4 genes and 1 isozyme of MnSOD. We isolated one of each of the green bamboo CuZnSOD and MnSOD genes, and their activities were stable under a broad range of pH and temperature treatments, even at room temperature for more than 3 days. Bamboo SODs showed developmental and tissue-specific regulation, and both transcript and protein levels were responsive to abscisic acid, UV-B and high-light treatments. The complexity of the cis-elements in promoter regions implied that the regulation mechanisms of SOD might help accomplish the unique fast-growth phenotype of green bamboo. © 2010 Elsevier Masson SAS.
Subjects
Abiotic stress; Antioxidant system; Bamboo; Bambusa oldhamii; Reactive oxygen species; Superoxide dismutase
Other Subjects
ascorbic acid; superoxide dismutase; vegetable protein; article; Bambusa; drug effect; enzyme assay; enzymology; light; metabolism; radiation exposure; reverse transcription polymerase chain reaction; ultraviolet radiation; Ascorbic Acid; Bambusa; Enzyme Assays; Light; Plant Proteins; Reverse Transcriptase Polymerase Chain Reaction; Superoxide Dismutase; Ultraviolet Rays; Bambusa; Dendrocalamopsis oldhamii
Type
journal article
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