Activation of the ERK signal transduction pathway by Epstein–Barr virus immediate-early protein Rta
Resource
Journal of General Virology 89 (10): 2437-2446
Journal
Journal of General
Journal Volume
89
Journal Issue
10
Pages
2437-2446
Date Issued
2008
Date
2008
Author(s)
Abstract
BRCA1-associated protein 2 (BRAP2) is known to interact with the kinase suppresson of Ras 1 (KSR1), inhibiting the ERK signal transduction cascade. This study found that an Epstein-Barr virus (EBV) immediate-early protein, Rta, is a binding partner of BRAP2 in yeast and confirmed the binding in vitro by a glutathione S-transferase pull-down assay and in vivo by coimmunoprecipitation in 293(maxi-EBV) cells. Binding studies also showed that Rta and KSR1 interacted with the C-terminal 202 aa region in BRAP2. Additionally, Rta appeared to prevent the binding of KSR1 to BRAP2, activating the ERK signal transduction pathway and the transcription of an EBV immediate-early gene, BZLF1. Activation of the ERK signal transduction pathway by Rta may be critical for the maintenance of the lytic state of EBV.
Publisher
Microbiology Society
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