Study on Mechanical Properties of Cancer Cells Using Magneto-Optical Tweezers
Date Issued
2009
Date
2009
Author(s)
Han, Chang-Mu
Abstract
Studying the intracellular interaction force is helpful for understanding the characteristics of cells. In recent years, it was found that cell shape plays an important role of controlling cell life and death. Therefore, the mechanical force comes from the interaction of cytoskeleton, which controls cell shape and cell motility, is worthy studying. In order to study the interaction within cells, nano(micro)-manipulation for cells is required. For example, AFM is used to analyze local mechanical properties of cells. In this thesis, we start from constructing and integrate magnetic tweezers with optical tweezers for manipulate small magnetic particles and specific cells. To explore the mechanism underlying for the mechanical properties of different compartments of cell, the mechanical equivalent models were applied to quantify the viscoelastic behavior of cells such as viscosity, elasticity and relaxation time. Depending on discussing these mechanical properties of different compartments of cell, it will help us to understand the difference of cell organelles in different conditions. In this thesis, human renal cancer cells were used to be examined and measured locally the mechanical properties of them by applying alternative magnetic field with 95 pN magnetic force. By analyzing the experimental data in terms of modified Voigt model, the cytoplasmic viscosity of the cancer cell is from 8.3 Pa?s to 39.3 Pa?s and the elasticity is from 3.7 Pa to 11 Pa. It was also found that the elasticity of the compartment close to cell membrane is 6-fold stiffer than the one of cytoplasm but viscosity of it is 2-fold less than the one of cytoplasm. In the end, magneto-optical tweezers is a potential technique for bioresearch and we can measure and analyze the mechanical properties of cells efficiently by using this system which can study the dynamic behavior of cells and provide novel information on biomedicine.
Subjects
magneto-optical tweezers
cytoskeleton
elasiticity
viscosity
SDGs
Type
thesis
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