Immunomodulatory Activities of Ganoderma lucidum Fermentation Products and Proteomic Analysis of Human Mononuclear Cells Following Treatment with Ganoderma lucidum Polysaccharide
Date Issued
2006
Date
2006
Author(s)
Yang, Shu-Chin
DOI
zh-TW
Abstract
Ganoderma lucidum is one of the most well known Chinese medicine in ancient times and is considered as the most valuable plant for health and longevity promotion. In this study, we investigated the effects of Ganoderma lucidum liquid fermentation broth on immunomodulatory activities in vitro and in vivo. After an in vitro experiment, we selected the most active fermentation product for experiments on non-specific immune response in BALB/c mice. In addition, we observed the MNC proliferation effect and performed the proteomic analysis of the protein expression by human mononuclear cells (MNC) treated with ethanol precipitated Ganoderma lucidum polysaccharide. Results show that Ganoderma lucidum fermentation product GL-93-12-21 stimulates human mononuclear cells to secrete cytokines and inhibit the human myelocytic lymphoma U937 cell growth. Moreover, the ethanol precipitated polysaccharide stimulats NO and TNF-α production in RAW 264.7 macrophage, and the polysaccharide characteristics are similar with bioactive polysaccharides that have been reported. In vivo studies showed that oral administration of Ganoderma lucidum fermentation product GL-93-12-21 on BALB/c mice significantly (P＜0.05) increases the secretion of IgG, the proliferation of T- and B-lymphocyte, the level of TNF-α、IL-2 and IFN-γ production, the phagocytosis effect of monocytes and the cytotoxicity of nature killer cells. Results also show that Ganoderma lucidum polysaccharide stimulates the proliferation of human mononuclear cells and changes the protein expression. There are 19 protein spots (1 down-regulated and 18 up-regulated) differentially expressed more than 1.5-fold, which was identified by quantitative image analysis and matrix assisted laser desorption ionization-quadrupole-time of flight (MALDI-Q-TOF) mass spectrometry. The Swiss-Prot database search indicated that increased expression of cytoskeleton and ribonucleoprotein is associated with the immune cell activation and proliferation. Furthermore, the increase of annexin A6 and chaperon protein expression, may play a part in activating of B lymphocyte and assembling of B cell receptor.
Subjects
靈芝
免疫調節
人類單核細胞
蛋白質體學
Ganoderma lucidum
Immunomodulatory
Human mononuclear cells
Proteomics
SDGs
Type
thesis
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