The Effects of Oral Delivery of Recombinant House Dust Mite Allergen on Airway Inflammation in Murine Model of Asthma
Date Issued
2009
Date
2009
Author(s)
Jian, Chien-Huei
Abstract
Asthma is one of the most common allergic diseases in children; in addition, about 80% of asthmatic patients in Taiwan are sensitized by house dust mite -- D. pteronyssinus. The characteristics of asthma such as AHR, eosinophils infiltration, antigen-specific T helper 2 cells activation, increased mucus secretion and even airway remodeling. Antigens-specific immune tolerance by prior oral administration of antigens might be a therapeutic strategy for allergic asthma. Therefore, we aimed to apply oral administration of rDp2 to decrease the airway inflammation induced by D. pteronyssinus. The female BALB/c mice were used and given with crude mite extract of D. pteronyssinus as the allergic asthma. In present study, we sensitized mice with peritoneal injection, and then challenged with intratracheal injection of crude mite extract. The results showed that peritoneal injection with high-dose could induce the clinical features of asthma significantly, including elevated mite-specific IgE in serum, production of Th2 cytokines (IL-5, IL-13) of splenocytes, and crude mite-specific lymphoproliferation. Furthermore, oral delivery of rDp2 or crude mite extract 0.2 or 1.0 mg/day for consecutive 7 days at the beginning of sensitization showed some beneficial effects on airway inflammation. Oral feeding 1.0 mg/day of rDp2 reduced AHR and slightly decreased the airway inflammation induced by crude mite extract. In conclusion, we suggest that oral delivery of high dose of single recombinant allergen seems to be more benefit on airway inflammation induced by the complex crude mite extract, and the feeding dose and feeding period need further investigation.
Subjects
allergic asthma
Dermatophagoides pteronyssinus
Dp2
oral tolerance
File(s)![Thumbnail Image]()
Loading...
Name
ntu-98-R96450005-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):a02a1350242b8ad666773dc8c07473b7