Study of sequence variation of dengue type 3 virus in naturally infected mosquitoes and human hosts: Implications for transmission and evolution
Journal
Journal of Virology
Journal Volume
78
Journal Issue
22
Pages
12717-12721
Date Issued
2004
Abstract
Dengue virus is an arbovirus that replicates alternately in the mosquito vector and human host. We investigated sequences of dengue type 3 virus in naturally infected Aedes aegypti mosquitoes and in eight patients from the same outbreak and reported that the extent of sequence variation seen with the mosquitoes was generally lower than that seen with the patients (mean diversity, 0.21 versus 0.38% and 0.09 versus 0.23% for the envelope [E] and capsid [C] genes, respectively). This was further verified with five experimentally infected mosquitoes (mean diversity, 0.09 and 0.10% for the E and C genes, respectively). Examination of the quasispecies structures of the E sequences of the mosquitoes and of the patients revealed that the sequences of the major variants were the same, suggesting that the major variant was transmitted. These findings support our hypothesis that mosquitoes contribute to the evolutionary conservation of dengue virus by maintaining a more homogenous viral population and a dominant variant during transmission.
SDGs
Other Subjects
Aedes aegypti; animal cell; article; clinical article; controlled study; Dengue virus; female; gene sequence; genetic conservation; genetic variability; human; human versus animal comparison; nonhuman; nucleotide sequence; priority journal; virus transmission; Aedes; Amino Acid Sequence; Animals; Base Sequence; Capsid Proteins; Conserved Sequence; Dengue; Dengue Virus; Evolution; Humans; Insect Vectors; Molecular Sequence Data; Viral Envelope Proteins
Type
journal article