Regulation of Gene Expression of Ghrelinin in a Rat Model of Myocardial Infarction
Date Issued
2006
Date
2006
Author(s)
Lin, Chien-Liang
DOI
zh-TW
Abstract
The acute myocardial infarction caused by coronary artery embolism is one of the common diseases around the world. A protein called ‘ghrelin’ was first isolated from rat stomach endocrine cells by kojima et al.in 1999 and its function of cardiac protection been proved found.It is a 28-amino-acid acylated peptide. It is found mainly in the stomach, pituitary and hypothalamus, but also found in numerous other tissues at lower levels. It could stimulate the release of growth hormone (GH) and also promote appetite and adiposity. In addition to other effects, it is involved in the regulation of energy balance and body weight homeostasis.
In addition, ghrelin also has many effects, such as inhibiting cardiomyocyte AraC-induced apoptosis, reducing cardiac afterload and increasing cardiac output without increasing heart rate in healthy volunteers, inducing vasodilation, improving the haemodynamics of patients with chronic heart failure (CHF), and regulating cardiovascular function in rats suffering septic shock.
According to previous studies, the fact that ghrelin has beneficial cardiovascular effects was confirmed. However, little is known about the regulation of ghrelin in response to myocardial infarction. The purpose of this study was to investigate the role of the ghrelin in myocardial infarction.
In this experiment, acute myocardial infarction ( AMI ) is going to be produced in 2.5 month-old male Wistar rats by ligating proximal left anterior descending ( LAD ) coronary artery with 6-0 silk suture. Rat cardiac ventricular tissues will be collected for analyszing the change of ghrelin gene to realize whether such a murine model can be a used as a model of AMI for ghrelin and evaluate the relationship between the chage of ghrelin and decline of cardiac function ?
After ligation of the left anterior descending coronary artery, expression of ghrelin significantly increased from 1 day to 4 weeks at left ventricle, and reached maximum at 5 days. Findings of Immuno- histochemical stain、hemodynamics and echocardiography can be confirmed the findings from Northern and Western blots. So it suggested that ghrelin could consist express even in AMI situation.
Gene therapy with ghrelin gene will be studied to see whether such a treatment is effective for myocardial infarction. This study may provide a fundamental basis for the development of more effective therapies in AMI treatment.
Subjects
冠狀動脈栓塞
急性心肌梗塞
Ghrelin
心臟血管
免疫組織染色
西方墨點轉印法
北方墨點轉印法
coronary artery embolism
acute myocardial infarction
ghrelin
cardiovascular system
Immunohistochemical stain
Northern blots
Western blots
Type
thesis
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