QTL Mapping for Cold Tolerance of Rice Seedling in the Cross between TNG67 and TN1 Varieties
Date Issued
2010
Date
2010
Author(s)
Dai, Hung-Yu
Abstract
To understand the genetic factors for chilling tolerance at rice seedling stage, a BC1 population from the cross between a cold-tolerant cultivar TNG67 (Oryza sativa L., cv. Tainung 67) and a cold-sensitive cultivar TN1 (Oryza sativa L., cv. Taichung Native 1) were developed for QTL analysis. TNG67 and TN1 were treated with low temperature (5℃) for 1, 3, 5 and 7 days and scored 7 days after returning to normal temperature (30/25℃), in order to find the biggest phenotypic difference for chilling tolerance between two parental varieties to augment the statistical power for QTL analysis. The biggest difference of survival rate between TNG67 and TN1 was observed when rice seedlings were treated with low temperature for 5 days. However, the F1 individuals showed significantly different phenotype from both parents. The F1 individuals survived but showed seriously retarded growth after the treatment of 5-days low temperature. Because the phenotype of the F1 individuals was expected in the BC1 mapping population, cold tolerance index and recovery rate, instead of survival rate, were used as phenotypic measurements for QTL mapping. In this study, 71 SSR markers were used to identify quantitative trait loci associated with recovery rate at seedling stage in 109 BC1 lines. A major QTL was identified on chromosome 11, named as qCT11, which explained 41.8％ of the phenotypic variation.
Subjects
rice
quantitative trait loci mapping
chilling
TNG67
TN1
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-99-R97621118-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):1272d20468acb93d037422995c66b2a8