The Effects of β-glucan on OVA-sensitizes Mice
Date Issued
2011
Date
2011
Author(s)
Lin, Wei-Cheng
Abstract
The aim of the experience is to understand mushroom polysaccharides can be prevented of allergy. The design of experiment continuous feeding different concentration of glucan for two weeks (0mg/200μl, 1mg/200μl, 2mg/200μl), and B6 mice were sensitized by OVA from twice to five times. Using different levels of sensitization to know the effect of polysaccharide feeding mice so we detected IFN-γ, IgG2a, IgE and IL-4 concentration in serum of the sensitization mice. Taking advantage of cytokines and immune globulin to study polysaccharides of the efficacy.
The results show that B6 mice sensitized several times, the group feeding mushroom polysaccharides of this experimental are eligible for a higher concentration of IFN-γ, and the group sensitized twice and four times by OVA, the experimental group and control group a comparison of significant differences (P <0.05). The IgG2a serum concentrations have similar results to IFN-γ, regardless of the sensitizion frequency,the group feeding glucan may be got higher IgG2a levels. The coparison of the polysaccharide treatment group and control group had a significant difference (P <0.05). The IgE concentrations in serum of feeding polysaccharides of B6 mice are lower than the control group, and the comparison of glucan treatmet and control had a significant difference (P <0.05) of the group sensitized twice to five times. The group fed 1mg/200μl of glucan had higher IL-4 concentrations in serum than any other treatment group or nontreayment froup, and there is significant difference (P <0.05). The result of IL-4 concentration in treatment group were lower than control group whether sensitization twice to five times, but no significant differences.
The results show that mushroom polysaccharides can effectively enhance the IFN-γ and IgG2a concentration in the blood and reduce IgE levels, amount regardless of the number of allegens.
Mushroom polysaccharides improved response to Th1cells, helps fight allergies, but the effects of IL-4 from mushroom polysaccharide is not obvious in this experiment.
Subjects
allergy
glucan
OVA
ovalbumin
Type
thesis
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