Development of an assay system for the analysis of host RISC activity in the presence of a potyvirus RNA silencing suppressor, HC-Pro
Journal
Virology journal
Journal Volume
20
Journal Issue
1
Date Issued
2023-01-17
Author(s)
Hong, Syuan-Fei
Fang, Ru-Ying
Wei, Wei-Lun
Jirawitchalert, Supidcha
Pan, Zhao-Jun
Hung, Yu-Ling
Pham, Thanh Ha
Chiu, Yen-Hsin
Shen, Tang-Long
Huang, Chien-Kang
Abstract
To investigate the mechanism of RNA silencing suppression, the genetic transformation of viral suppressors of RNA silencing (VSRs) in Arabidopsis integrates ectopic VSR expression at steady state, which overcomes the VSR variations caused by different virus infections or limitations of host range. Moreover, identifying the insertion of the transgenic VSR gene is necessary to establish a model transgenic plant for the functional study of VSR.
Subjects
AGO1 degradation; HC-pro; In vitro RISC assay; T-DNA insertion
Publisher
BMC
Type
journal article