Repository logo
  • English
  • 中文
Log In
Have you forgotten your password?
  1. Home
  2. College of Life Science / 生命科學院
  3. Plant Biology / 植物科學研究所
  4. Molecular cloning of Oncidium lip-development related genes that are disturbed by high temperature
 
  • Details

Molecular cloning of Oncidium lip-development related genes that are disturbed by high temperature

Date Issued
2004
Date
2004
Author(s)
Lin, Li-Chieh
DOI
zh-TW
URI
http://ntur.lib.ntu.edu.tw//handle/246246/57962
Abstract
Oncidium Grower Ramsey is one of major cut flower species in Taiwan, and the flower production is all over the year. However, high temperature of summer and autumn results in the poor quality of cut flowers, by that not only the branching and floral number of inflorescence drop severely, but the length of inflorescence is shorter than that developed in cool seasons. Besides, high temperature might cause the floral lips smaller and slightly deformed. I focused my study in the cloning of floral development-related genes that are interfered by high temperature. Pot plants with developing inflorescences were cultivated in growth chambers setting at regular (28/23℃) or high temperature (35/30℃), and floral lips at various developmental stages were collected for RNA extraction. By using DDRT-PCR technique, 1323 gene fragments shown differential expression were collected and 324 of them were cloned and sequenced. After Blast alignment, ten clones possessing high homology with known genes related to cell wall metabolism and cell expansion were selected and used as probes to examine the gene expression pattern during floral development. The expression of six genes(OACS, OPHAN1, OV-ATPase, OKTN, OExp1 and OAuxRFP)in lip tissues increased along with the normal development of floral bud; and high temperature hampered their gene activities. The expression of other four genes(OASD, OCCR, OEGase and OGA2ox)which were selected from high temperature-treated lips was confirmed to be enhanced by high temperature. OGA2ox was predicted to encode GA-2-oxidase which is a well-known enzyme to regulate the level of biologically active GAs in plant tissues. Although the GA content in floral lips were not determined, exogenous GA application to floral buds grown at high temperature was conducted to test the effect of GA on lip development. The results suggested that GA should be involved in the regulation of lip expansion.
Subjects
荷爾蒙
唇瓣
lip
high temperature
Oncidium
GA
Type
other
File(s)
Loading...
Thumbnail Image
Name

ntu-93-R90226021-1.pdf

Size

23.31 KB

Format

Adobe PDF

Checksum

(MD5):485c39d6853174c962ab7b3d2a5c5513

臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

總館學科館員 (Main Library)
醫學圖書館學科館員 (Medical Library)
社會科學院辜振甫紀念圖書館學科館員 (Social Sciences Library)

開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

  • 請確認所上傳的全文是原創的內容,若該文件包含部分內容的版權非匯入者所有,或由第三方贊助與合作完成,請確認該版權所有者及第三方同意提供此授權。
    Please represent that the submission is your original work, and that you have the right to grant the rights to upload.
  • 若欲上傳已出版的全文電子檔,可使用Open policy finder網站查詢,以確認出版單位之版權政策。
    Please use Open policy finder to find a summary of permissions that are normally given as part of each publisher's copyright transfer agreement.
  • 網站簡介 (Quickstart Guide)
  • 使用手冊 (Instruction Manual)
  • 線上預約服務 (Booking Service)
  • 方案一:臺灣大學計算機中心帳號登入
    (With C&INC Email Account)
  • 方案二:ORCID帳號登入 (With ORCID)
  • 方案一:定期更新ORCID者,以ID匯入 (Search for identifier (ORCID))
  • 方案二:自行建檔 (Default mode Submission)
  • 方案三:學科館員協助匯入 (Email worklist to subject librarians)

Built with DSpace-CRIS software - Extension maintained and optimized by 4Science