Precise Control of Intracellular Trafficking and Receptor-Mediated Endocytosis in Living Cells and Behaving Animals
Journal
Advanced Science
Journal Issue
2405568
ISSN
2198-3844
Date Issued
2024-10-14
Author(s)
Shiau‐Chi Chen
Neng‐Jie Zeng
Grace Y. Liu
Hsien‐Chu Wang
Tzu‐Ying Lin
Yi‐Ling Tai
Chiao‐Yun Chen
Yin Fang
Yi‐Chien Chuang
Ching‐Lin Kao
Hsuan Cheng
Bing‐Huang Wu
Pin‐Chiao Sun
Odvogmed Bayansan
Yu‐Ting Chiu
Chi‐Hsuan Shih
Wen‐Hong Chung
Jia‐Bin Yang
Lily Hui‐Ching Wang
Po‐Han Chiang
Oliver I. Wagner
Yi‐Ching Wang
Yu‐Chun Lin
DOI
10.1002/advs.202405568
Abstract
Intracellular trafficking, an extremely complex network, dynamically orchestrates nearly all cellular activities. A versatile method that enables the manipulation of target transport pathways with high spatiotemporal accuracy in vitro and in vivo is required to study how this network coordinates its functions. Here, a new method called RIVET (Rapid Immobilization of target Vesicles on Engaged Tracks) is presented. Utilizing inducible dimerization between target vesicles and selective cytoskeletons, RIVET can spatiotemporally halt numerous intracellular trafficking pathways within seconds in a reversible manner. Its highly specific perturbations allow for the real-time dissection of the dynamic relationships among different trafficking pathways. Moreover, RIVET is capable of inhibiting receptor-mediated endocytosis. This versatile system can be applied from the cellular level to whole organisms. RIVET opens up new avenues for studying intracellular trafficking under various physiological and pathological conditions and offers potential strategies for treating trafficking-related disorders.
Subjects
chemical-inducible dimerization
cytoskeleton
optogenetics
receptor-mediated endocytosis
vesicular trafficking
virus entry
Publisher
Wiley
Description
Online Version of Record before inclusion in an issue 2405568
Type
journal article