Functional Studies of Glutathione S-TransferaseAtGSTU17)and Phytochrome A in Response to Light,Hormones and Stress Conditions
Date Issued
2009
Date
2009
Author(s)
Jiang, Han-Vei
Abstract
Phytochrome A (phyA) is the photoreceptor of far-red (FR) light. Previoustudies have shown that a glutathione S-transferase (AtGSTU17), a tau classember of the GST gene family in Arabidopsis, can be induced rapidly in wildype under FR light, but abolished in the phyA mutant. The molecularechanisms underlying the regulation of GSTU17 expression by phyA remainnknown. We isolated the gstu17phyA double mutant showing that it exhibited aonger hypocotyl phenotype than its parental lines specifically under weak FRight. When AtGSTU17 was overexpressed in the phyA mutant backgroundGSTU17OE-2/phyA), it led to a shorter hypocotyl phenotype than the phyAnder FR light. These data suggest that AtGSTU17 and PHYA act as a nonalleliconcomplementary relation, and may work in parallel pathways or physicallynteract with each other to control hypocotyl elongation under FR. Furthermore,T-PCR analyses indicated that several auxin responsive genes werep-regulated, and phytochrome-interacting factors (PIFs) as well as lightesponsive gene such as CHS down-regulated in the gstu17 mutant under FR. Inddition, several ABA-regualted genes were affected in the gstu17 mutant.nder the ionic stress and osmotic stress treatments, the gstu17 mutant isyposensitive in terms of lateral roots inhibition. Taken together, these datandicate that AtGSTU17 may function as a crosstalk among phyA-mediated FRight, auxin, ABA and stress signaling pathways to regulate hypocotyl elongationnd root development in Arabidopsis.
Subjects
Arabidopsis
phytochrome
ABA
Far-red
GST
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