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Utilizing Multiphoton Microscopy to Monitor Chemically Enhanced Transdermal Delivery Pathways of Luminescent Quantum Dots
Date Issued
2004
Date
2004
Author(s)
Lo, Wen
DOI
en-US
Abstract
Luminescent quantum dots (QDs) are attractive fluorescent probes in bioimaging application. Broad excitation spectrum, narrow and tunable emission spectrum, high photochemical stability and chemical modifiability are among the desirable properties of QDs. In this work, we investigate the feasibility of administering QDs (7 nm in size) through transdermal delivery pathway using multiphoton fluorescence imaging. Our results show that QDs of this size range can be effectively delivered through intercellular pathways using the chemical enhancer oleic acid. This work has implications for understanding the transport of important biological macromolecules.
Our experiments infer two important results. First QDs can hardly transport into skin without the chemical enhancer oleic acid. Furthermore, QDs deliver primarily through intercellular pathways. In contrast, fluoresein molecules deliver through both intercellular and intracellular region.
Our results imply that the size of nanoparticles may dominate the nature of transdermal transport mechanism and in the future, we plan to investigate the mechanism by manipulating the size of nanoparticles and their surface chemistry.
Our experiments infer two important results. First QDs can hardly transport into skin without the chemical enhancer oleic acid. Furthermore, QDs deliver primarily through intercellular pathways. In contrast, fluoresein molecules deliver through both intercellular and intracellular region.
Our results imply that the size of nanoparticles may dominate the nature of transdermal transport mechanism and in the future, we plan to investigate the mechanism by manipulating the size of nanoparticles and their surface chemistry.
Subjects
量子點
雙光子
皮膚
脈衝雷射
顯微術
油酸
藥物傳遞
multiphoton
skin
chemically enhanced
quantum dots
microscopy
two-photon
oleic acid
transdermal delivery
ti-sapphire laser
Type
thesis
File(s)
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Name
ntu-93-R92222031-1.pdf
Size
23.53 KB
Format
Adobe PDF
Checksum
(MD5):61f459414ff20395bcc2a456cf48c319